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MiR-23a facilitates the replication of HSV-1 through the suppression of interferon regulatory factor 1
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 12, p e114021 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression. It has been reported that miRNAs are involved in host-virus interaction, but evidence that cellular miRNAs promote virus replication has been limited. Here, we found that miR-23a promoted the replication of human herpes simplex virus type 1 (HSV-1) in HeLa cells, as demonstrated by a plaque-formation assay and quantitative real-time PCR. Furthermore, interferon regulatory factor 1 (IRF1), an innate antiviral molecule, is targeted by miR-23a to facilitate viral replication. MiR-23a binds to the 3'UTR of IRF1 and down-regulates its expression. Suppression of IRF1 expression reduced RSAD2 gene expression, augmenting HSV-1 replication. Ectopic expression of IRF1 abrogated the promotion of HSV-1 replication induced by miR-23a. Notably, IRF1 contributes to innate antiviral immunity by binding to IRF-response elements to regulate the expression of interferon-stimulated genes (ISGs) and apoptosis, revealing a complex interaction between miR-23a and HSV-1. MiR-23a thus contributes to HSV-1 replication through the regulation of the IRF1-mediated antiviral signal pathway, which suggests that miR-23a may represent a promising target for antiviral treatments.
- Subjects :
- Expression of Concern
Oxidoreductases Acting on CH-CH Group Donors
Lytic Cycle
lcsh:Medicine
Herpesvirus 1, Human
Virus Replication
Microbiology
Virus Effects on Host Gene Expression
DNA replication factor CDT1
Virology
Molecular Cell Biology
microRNA
Gene expression
Humans
lcsh:Science
Molecular Biology
Gene
Regulation of gene expression
Multidisciplinary
biology
lcsh:R
Proteins
Biology and Life Sciences
Cell Biology
Viral Replication
Cell biology
MicroRNAs
IRF1
Gene Expression Regulation
Viral replication
Host-Pathogen Interactions
biology.protein
lcsh:Q
Ectopic expression
HeLa Cells
Interferon Regulatory Factor-1
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....fd6adb94add22363e637d53f0523972e