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Rearrangements of Blood and Tissue Fatty Acid Profile in Colorectal Cancer - Molecular Mechanism and Diagnostic Potential
- Source :
- Frontiers in Oncology, Frontiers in Oncology, Vol 11 (2021)
- Publication Year :
- 2021
-
Abstract
- Colorectal cancer (CRC) is often diagnosed at an advanced stage due to the invasiveness of colonoscopy; thus, non-invasive CRC diagnostics are desirable. CRC is associated with lipid alterations. We aimed to verify whether fatty acid (FA) profiles in CRC patients may serve as a potential diagnostic tool for CRC diagnosis. FA profiles were assayed by GC-MS in cancer tissue, paired normal mucosa and serum from CRC patients and healthy controls. The levels of very long FAs – VLCFAs (26:0, 28:0 and 26:1) were the most highly increased FAs in cancer tissue compared to normal colon mucosa. Moreover, these FA were present in serum of CRC patients, they were absent in the serum of healthy subjects, or present in only trace amounts. To verify if cancer cells are the source of small amounts of these VLCFAs in the serum of patients we performed experiment in HT-29 CRC cells, which proved that CRC cells can produce and release VLCFAs into the blood. Most importantly, we defined a panel of FAs that may be assayed in a single analysis that definitely distinguishes CRC patients and healthy subjects, which was confirmed by PLS-DA and multivariate ROC analysis (AUC = 0.985). This study shows that selected FA panel may serve as a diagnostic marker for CRC.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Cancer Research
Colorectal cancer
Normal colon
Colonoscopy
colorectal cancer
PLS-DA
Gastroenterology
lipids
03 medical and health sciences
0302 clinical medicine
Internal medicine
medicine
neoplasms
RC254-282
Original Research
chemistry.chemical_classification
PCA
medicine.diagnostic_test
business.industry
Fatty acid
Cancer
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Diagnostic marker
medicine.disease
digestive system diseases
gas chromatography-mass spectrometry
030104 developmental biology
chemistry
Oncology
030220 oncology & carcinogenesis
Cancer cell
Molecular mechanism
fatty acid
business
Subjects
Details
- ISSN :
- 2234943X
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Frontiers in oncology
- Accession number :
- edsair.doi.dedup.....fd6b229010010a1e898d387e619311be