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Pharmacokinetic study of traditional Japanese Kampo medicine shimotsuto used to treat gynecological diseases in rats
- Source :
- Journal of Natural Medicines
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Abstract Shimotsuto is a traditional Japanese Kampo medicine used to treat gynecological diseases, such as irregular menstruation, in addition to oversensitivity to cold and chilblains. Part of the pharmacological actions of shimotsuto is traditionally considered to be exerted by an improvement effect of the blood and the circulatory system. Multiple ingredients (e.g., catalpol and paeoniflorin) contained in shimotsuto have been reported to have pharmacological activities on the blood and circulatory system, and thus been considered to contribute to the pharmacological actions of shimotsuto. However, it remains unclear whether the ingredients can be absorbed into the body following oral administration of shimotsuto. The aim in the present study was to specify shimotsuto ingredient absorbed into the systemic circulation in rats. Seven candidate active ingredients (catalpol, paeoniflorin, albiflorin, ligustilide, senkyunolide A, butylphthalide, and ferulic acid) in plasma after oral administration of shimotsuto were quantified by targeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis. This study also performed nontargeted LC–MS/MS analysis of plasma following administration of constituent crude drugs of shimotsuto to find extensively blood-absorbed ingredients of shimotsuto. Among detected peaks in the nontargeted analysis, two peaks could be identified as bergapten and 8-debenzoylpaeoniflorin, subsequently their concentrations in shimotsuto-treated rat plasma were quantified. These pharmacokinetic studies indicated that catalpol showed the highest plasma concentration following administration of shimotsuto, followed by 8-debenzoylpaeoniflorin. This study suggests that all nine ingredients are absorbed into the blood following oral administration of shimotsuto and possibly contribute to its pharmacological action. Graphic abstract
- Subjects :
- Male
Kampo
Pharmacology
Bergapten
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Japan
Pharmacokinetics
Tandem Mass Spectrometry
Oral administration
Animals
Medicine
Shimotsuto
Gynecological disease
Active ingredients
Chromatography, High Pressure Liquid
030304 developmental biology
Active ingredient
Original Paper
0303 health sciences
business.industry
Paeoniflorin
Catalpol
Rats
Butylphthalide
chemistry
Blood and circulatory system
Molecular Medicine
Female
Medicine, Kampo
business
Genital Diseases, Female
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 18610293 and 13403443
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- Journal of Natural Medicines
- Accession number :
- edsair.doi.dedup.....fd7d8c01c27981c116605dacfcceb57e