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TBX2 and TBX3 act downstream of canonical WNT signaling in patterning and differentiation of the mouse ureteric mesenchyme
- Source :
- Development (Cambridge, England), 145(23). Company of Biologists Ltd
- Publication Year :
- 2018
-
Abstract
- The organized array of smooth muscle cells (SMCs) and fibroblasts in the walls of visceral tubular organs arises by patterning and differentiation of mesenchymal progenitors surrounding the epithelial lumen. Here, we show that the TBX2 and TBX3 transcription factors have novel and required roles in regulating these processes in the murine ureter. Co-expression of TBX2 and TBX3 in the inner mesenchymal region of the developing ureter requires canonical WNT signaling. Loss of TBX2/TBX3 in this region disrupts activity of two crucial drivers of the SMC program, Foxf1 and BMP4 signaling, resulting in decreased SMC differentiation and increased extracellular matrix. Transcriptional profiling and chromatin immunoprecipitation experiments revealed that TBX2/TBX3 directly repress expression of the WNT antagonists Dkk2 and Shisa2, the BMP antagonist Bmper and the chemokine Cxcl12. These findings suggest that TBX2/TBX3 are effectors of canonical WNT signaling in the ureteric mesenchyme that promote SMC differentiation by maintaining BMP4 and WNT signaling in the inner region, while restricting CXCL12 signaling to the outer layer of fibroblast-fated mesenchyme.
- Subjects :
- 0301 basic medicine
Chemokine
Mesenchyme
Myocytes, Smooth Muscle
Bone Morphogenetic Protein 4
Models, Biological
Extracellular matrix
Mesoderm
03 medical and health sciences
Mice
medicine
Animals
Molecular Biology
Transcription factor
Wnt Signaling Pathway
Body Patterning
biology
Effector
Mesenchymal stem cell
Wnt signaling pathway
Gene Expression Regulation, Developmental
Cell Differentiation
Embryo, Mammalian
Cell biology
030104 developmental biology
medicine.anatomical_structure
biology.protein
Peristalsis
Ureter
T-Box Domain Proteins
Transcriptome
Chromatin immunoprecipitation
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 09501991
- Volume :
- 145
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- Development (Cambridge, England)
- Accession number :
- edsair.doi.dedup.....fdab11bef7b6c6da40ec38c310e52ed4