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Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage
- Source :
- Scientific Reports, Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Heart failure (HF) and the development of chronic kidney disease (CKD) have a direct association. Both can be cause and consequence of the other. Many factors are known, such as diabetes or hypertension, which can lead to the appearance and/or development of these two conditions. However, it is suspected that other factors, namely genetic ones, may explain the differences in the manifestation and progression of HF and CKD among patients. One candidate factor is Rph, a gene expressed in the nervous and excretory system in mammals and Drosophila, encoding a Rab small GTPase family effector protein implicated in vesicular trafficking. We found that Rph is expressed in the Drosophila heart, and the silencing of Rph gene expression in this organ had a strong impact in the organization of fibers and functional cardiac parameters. Specifically, we observed a significant increase in diastolic and systolic diameters of the heart tube, which is a phenotype that resembles dilated cardiomyopathy in humans. Importantly, we also show that silencing of Rabphilin (Rph) expression exclusively in the pericardial nephrocytes, which are part of the flies' excretory system, brings about a non-cell-autonomous effect on the Drosophila cardiac system. In summary, in this work, we demonstrate the importance of Rph in the fly cardiac system and how silencing Rph expression in nephrocytes affects the Drosophila cardiac system.
- Subjects :
- Cardiomyopathy, Dilated
0301 basic medicine
Physiology
Science
Nerve Tissue Proteins
030204 cardiovascular system & hematology
Biology
Article
03 medical and health sciences
0302 clinical medicine
Developmental biology
Gene expression
Genetics
medicine
Animals
Drosophila Proteins
Gene silencing
Small GTPase
Gene Silencing
Multidisciplinary
Effector
fungi
Dilated cardiomyopathy
medicine.disease
Phenotype
Cell biology
030104 developmental biology
Heart failure
Medicine
Rab
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....fdb672f0c1ba5e8bdf6068ceb584e2b5