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Two clones obtained from Urabe AM9 mumps virus vaccine differ in their replicative efficiency in neuroblastoma cells
- Source :
- Microbes and Infection. 8:332-339
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- A high rate of post-vaccinal aseptic meningitis for Urabe AM9 mumps virus strain is well documented. This strain is composed of two virus variants differing at the nt 1081 (A/G) region in the hemagglutinin-neuraminidase (HN) gene. An association of HN-A(1081) variant with neurovirulence has been proposed. In order to test for neurotropism we isolated the HN-A(1081) and HN-G(1081) virus variants from Urabe AM9 mumps virus vaccine. Sequential passages were performed in monkey kidney Vero cells and human neuroblastoma SH-SY5Y cells. Viral replication was determined by conventional and real-time RT-PCR. The results show that clone HN-A(1081) can replicate efficiently in both cell types. However, a defective replication of clone HN-G(1081), lacking its genetic marker, was observed after the third passage in neuroblastoma cells. Kinetics assays showed that clone HN-A(1081) replicates faster than clone HN-G(1081). Viral clones were also inoculated into the brains of newborn rats. Clone HN-A(1081) replicated 14 times, while clone HN-G(1081) merely duplicated its level over the initial inoculum. These results suggest that there is a selective replication of HN-A(1081) mumps virus variants in cells of nervous origin.
- Subjects :
- Paramyxoviridae
Immunology
Clone (cell biology)
Mumps Vaccine
Mumps virus
Biology
Virus Replication
medicine.disease_cause
Microbiology
Virus
Cell Line
Neuroblastoma
Cell Line, Tumor
Chlorocebus aethiops
medicine
Animals
Humans
Point Mutation
Cloning, Molecular
Rubulavirus
Mononegavirales
Mumps
Vero Cells
HN Protein
Brain
biology.organism_classification
Virology
Rats
Infectious Diseases
Animals, Newborn
Viral replication
Hemagglutinin-neuraminidase
Subjects
Details
- ISSN :
- 12864579
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Microbes and Infection
- Accession number :
- edsair.doi.dedup.....fdcefc629f0de0601e6c20296b7a2bc6