Back to Search
Start Over
Immunization of Malaria-Preexposed Volunteers With PfSPZ Vaccine Elicits Long-Lived IgM Invasion-Inhibitory and Complement-Fixing Antibodies
- Source :
- The Journal of infectious diseases. 217(10)
- Publication Year :
- 2017
-
Abstract
- Background The assessment of antibody responses after immunization with radiation-attenuated, aseptic, purified, cryopreserved Plasmodium falciparum sporozoites (Sanaria PfSPZ Vaccine) has focused on IgG isotype antibodies. Here, we aimed to investigate if P. falciparum sporozoite binding and invasion-inhibitory IgM antibodies are induced following immunization of malaria-preexposed volunteers with PfSPZ Vaccine. Methods Using serum from volunteers immunized with PfSPZ, we measured vaccine-induced IgG and IgM antibodies to P. falciparum circumsporozoite protein (PfCSP) via ELISA. Function of this serum as well as IgM antibody fractions was measured via in vitro in an inhibition of sporozoite invasion assay. These IgM antibody fractions were also measured for binding to sporozoites by immunofluorescence assay and complement fixation on whole sporozoites. Results We found that in addition to anti-PfCSP IgG, malaria-preexposed volunteers developed anti-PfCSP IgM antibodies after immunization with PfSPZ Vaccine and that these IgM antibodies inhibited P. falciparum sporozoite invasion of hepatocytes in vitro. These IgM plasma fractions also fixed complement to whole P. falciparum sporozoites. Conclusions This is the first finding that PfCSP and P. falciparum sporozoite-binding IgM antibodies are induced following immunization of PfSPZ Vaccine in malaria-preexposed individuals and that IgM antibodies can inhibit P. falciparum sporozoite invasion into hepatocytes in vitro and fix complement on sporozoites. These findings indicate that the immunological assessment of PfSPZ Vaccine-induced antibody responses could be more sensitive if they include parasite-specific IgM in addition to IgG antibodies. Clinical trials registration NCT02132299.
- Subjects :
- 0301 basic medicine
Adult
Male
Volunteers
Plasmodium falciparum
Antibodies, Protozoan
Vaccines, Attenuated
Immunoglobulin G
03 medical and health sciences
Young Adult
Major Articles and Brief Reports
0302 clinical medicine
Double-Blind Method
parasitic diseases
Malaria Vaccines
Immunology and Allergy
Medicine
Humans
030212 general & internal medicine
Malaria, Falciparum
biology
business.industry
Vaccination
Complement fixation test
biology.organism_classification
Virology
PfSPZ vaccine
Malaria
Circumsporozoite protein
030104 developmental biology
Infectious Diseases
Immunization
Immunoglobulin M
Sporozoites
Antibody Formation
biology.protein
Antibody
business
Subjects
Details
- ISSN :
- 15376613
- Volume :
- 217
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- The Journal of infectious diseases
- Accession number :
- edsair.doi.dedup.....fdece9b50caaf82f30ddb5b22cb1afa4