Back to Search
Start Over
Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens
- Source :
- Steffensen, R, Baech, J & Nielsen, K R 2015, Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens . in P Bugert (ed.), Molecular Typing of Blood Cell Antigens . Springer, Methods in Molecular Biology, vol. 1310, pp. 205-212 . https://doi.org/10.1007/978-1-4939-2690-9_17, Molecular Typing of Blood Cell Antigens ISBN: 9781493926893
- Publication Year :
- 2015
- Publisher :
- Springer, 2015.
-
Abstract
- Neutrophil antigens are implicated in a variety of clinical conditions, including neonatal immune neutropenia, transfusion-related acute lung injury, refractoriness to granulocyte transfusions, febrile transfusion reactions, and autoimmune neutropenia. In this report, we describe simultaneous genotyping of human neutrophil antigens (HNA)-1, -3, -4, and -5 using PCR with allele-specific TaqMan probes and end-point fluorescence detection, which is a robust, rapid, and reproducible method, allowing for high-throughput genotyping.
Details
- Language :
- English
- ISBN :
- 978-1-4939-2689-3
- ISBNs :
- 9781493926893
- Database :
- OpenAIRE
- Journal :
- Steffensen, R, Baech, J & Nielsen, K R 2015, Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens . in P Bugert (ed.), Molecular Typing of Blood Cell Antigens . Springer, Methods in Molecular Biology, vol. 1310, pp. 205-212 . https://doi.org/10.1007/978-1-4939-2690-9_17, Molecular Typing of Blood Cell Antigens ISBN: 9781493926893
- Accession number :
- edsair.doi.dedup.....fdf0a7ccf04cf5d40d2200cd058a51d9
- Full Text :
- https://doi.org/10.1007/978-1-4939-2690-9_17