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High BrdU-sensitivity of Passeriformes chromosomes: conservation of BrdU- sensitive fragile sites on their Z chromosomes during evolution
- Source :
- Cytogenetic and Genome Research, Cytogenetic and Genome Research, Karger, 2019, ⟨10.1159/000499590⟩, Cytogenetic and Genome Research, Karger, 2019, 157 (3), pp.158-165. ⟨10.1159/000499590⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- Amongst 15 bird species, representative of 7 orders, recurrent breakages evocating the presence of fragile sites were detected in the chromosomes of the 5 species belonging to Passeriformes. These breaks appeared when 5-bromodeoxyuridine (BrdU) was added to the cell culture medium at a dose inefficient for inducing chromosome structure alterations in other birds and mammals. They involved, similarly in male and female, 3 loci on the Z chromosome of 3 Turdus species (Turdidae). Labeling by BrdU antibody confirmed the correlation between BrdU incorporation into DNA and breakage, especially around and in the sites of breakage. Thus, 3 BrdU-sensitive fragile sites were present in the Z chromosomes of these birds. Three fragile sites were also detected at different locations in the Z chromosomes of the European robin (Erithacus rubecula, Muscicapidae), suggesting that a structural rearrangement occurred during the evolution of Turdidae and Muscicapidae. Chromosome banding confirmed this interpretation. Finally, in the more distantly related species Parus major (Paridae), the almost acrocentric Z chromosome displayed a single BrdU-sensitive fragile site in its short arm, and the W appeared to be pulverized by BrdU incorporation. Although it cannot be excluded that the BrdU-sensitive fragile sites may be involved in rearrangements, their conservation in many species, and possibly all Passeriformes, provides evidence that they do not constitute a pejorative character during evolution.
- Subjects :
- Male
[SDV]Life Sciences [q-bio]
European robin
Chromosomes
Evolution, Molecular
03 medical and health sciences
chemistry.chemical_compound
biology.animal
Centromere
Genetics
Animals
Passeriformes
Molecular Biology
Genetics (clinical)
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Parus
0303 health sciences
Z chromosome
Base Composition
[SDV.GEN]Life Sciences [q-bio]/Genetics
biology
Chromosomal fragile site
Chromosome Fragile Sites
030302 biochemistry & molecular biology
Chromosome
Karyotype
biology.organism_classification
Chromosome Banding
chemistry
Bromodeoxyuridine
Evolutionary biology
Female
DNA
Subjects
Details
- Language :
- English
- ISSN :
- 14248581 and 1424859X
- Database :
- OpenAIRE
- Journal :
- Cytogenetic and Genome Research, Cytogenetic and Genome Research, Karger, 2019, ⟨10.1159/000499590⟩, Cytogenetic and Genome Research, Karger, 2019, 157 (3), pp.158-165. ⟨10.1159/000499590⟩
- Accession number :
- edsair.doi.dedup.....fdf545a83b5bb72b56b3bc2315baa792