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Isolation of Genetically Diverse Marburg Viruses from Egyptian Fruit Bats
- Source :
- PLoS Pathogens, PLoS Pathogens, Vol 5, Iss 7, p e1000536 (2009)
- Publication Year :
- 2009
- Publisher :
- Public Library of Science, 2009.
-
Abstract
- In July and September 2007, miners working in Kitaka Cave, Uganda, were diagnosed with Marburg hemorrhagic fever. The likely source of infection in the cave was Egyptian fruit bats (Rousettus aegyptiacus) based on detection of Marburg virus RNA in 31/611 (5.1%) bats, virus-specific antibody in bat sera, and isolation of genetically diverse virus from bat tissues. The virus isolates were collected nine months apart, demonstrating long-term virus circulation. The bat colony was estimated to be over 100,000 animals using mark and re-capture methods, predicting the presence of over 5,000 virus-infected bats. The genetically diverse virus genome sequences from bats and miners closely matched. These data indicate common Egyptian fruit bats can represent a major natural reservoir and source of Marburg virus with potential for spillover into humans.<br />Author Summary Marburg virus, similar to its close cousin Ebola virus, can cause large outbreaks of hemorrhagic fever (HF) in rural Africa with case fatalities approaching 90%. For decades, a long-standing enigma has been the identity of the natural reservoir of this deadly virus. In this report, we identify the cave-dwelling Egyptian fruit bat (Rousettus aegyptiacus) as a natural host of Marburg virus based on multiple lines of evidence which include, for the first time ever, the isolation of virus directly from wild-caught and apparently healthy bats. The species R. aegyptiacus is common throughout Africa with distribution into the eastern Mediterranean and Middle East. Our finding of active virus infection in approximately 5% of R. aegyptiacus bats and their population exceeding 100,000 in Kitaka cave in Uganda suggests there are likely over 5,000 Marburg virus–infected bats in this cave, which is only one of many such cave populations throughout Africa. Clearly, these bats could serve as a major source of virus with potential to initiate human epidemics, and the implications for public health are striking. Additionally, we found highly divergent (21%) genome sequences among viruses circulating in these bat populations, a level of diversity that would result from a long-term association with a suitable reservoir host of large population size.
- Subjects :
- Male
animal structures
QH301-705.5
viruses
Immunology
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
Antibodies, Viral
Microbiology
Virus
Virology/Emerging Viral Diseases
Marburg virus
Marburg virus disease
Virology
Chiroptera
Genetics
medicine
Animals
Humans
Natural reservoir
Marburg Virus Disease
Uganda
Biology (General)
Molecular Biology
Antigens, Viral
Phylogeny
Ebola virus
Lloviu virus
biology
Reverse Transcriptase Polymerase Chain Reaction
RC581-607
biology.organism_classification
Marburgvirus
Immunohistochemistry
Liver
Parasitology
Female
Immunologic diseases. Allergy
Rousettus
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 15537374 and 15537366
- Volume :
- 5
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS Pathogens
- Accession number :
- edsair.doi.dedup.....fe7f698b1f003b883bdbdc697f81a33d