Back to Search Start Over

Isolation of Genetically Diverse Marburg Viruses from Egyptian Fruit Bats

Authors :
Thomas G. Ksiazek
Alan Kemp
Patrick Atimnedi
David M. Miller
Marina L. Khristova
John Kayiwa
Robert Downing
Pierre Formenty
Tara K. Sealy
Jonathan S. Towner
James A. Comer
Emmanuel Byaruhanga
Edward Katongole-Mbidde
Stephen Balinandi
César G. Albariño
James N. Mills
Sherif R. Zaki
Zachary Reed
Stuart T. Nichol
Samuel Okware
Jordan W. Tappero
Robert Swanepoel
Eileen C. Farnon
Pierre E. Rollin
Patricia W. Greer
Deborah Cannon
Brian R. Amman
Serena A. Carroll
Christopher D. Paddock
Source :
PLoS Pathogens, PLoS Pathogens, Vol 5, Iss 7, p e1000536 (2009)
Publication Year :
2009
Publisher :
Public Library of Science, 2009.

Abstract

In July and September 2007, miners working in Kitaka Cave, Uganda, were diagnosed with Marburg hemorrhagic fever. The likely source of infection in the cave was Egyptian fruit bats (Rousettus aegyptiacus) based on detection of Marburg virus RNA in 31/611 (5.1%) bats, virus-specific antibody in bat sera, and isolation of genetically diverse virus from bat tissues. The virus isolates were collected nine months apart, demonstrating long-term virus circulation. The bat colony was estimated to be over 100,000 animals using mark and re-capture methods, predicting the presence of over 5,000 virus-infected bats. The genetically diverse virus genome sequences from bats and miners closely matched. These data indicate common Egyptian fruit bats can represent a major natural reservoir and source of Marburg virus with potential for spillover into humans.<br />Author Summary Marburg virus, similar to its close cousin Ebola virus, can cause large outbreaks of hemorrhagic fever (HF) in rural Africa with case fatalities approaching 90%. For decades, a long-standing enigma has been the identity of the natural reservoir of this deadly virus. In this report, we identify the cave-dwelling Egyptian fruit bat (Rousettus aegyptiacus) as a natural host of Marburg virus based on multiple lines of evidence which include, for the first time ever, the isolation of virus directly from wild-caught and apparently healthy bats. The species R. aegyptiacus is common throughout Africa with distribution into the eastern Mediterranean and Middle East. Our finding of active virus infection in approximately 5% of R. aegyptiacus bats and their population exceeding 100,000 in Kitaka cave in Uganda suggests there are likely over 5,000 Marburg virus–infected bats in this cave, which is only one of many such cave populations throughout Africa. Clearly, these bats could serve as a major source of virus with potential to initiate human epidemics, and the implications for public health are striking. Additionally, we found highly divergent (21%) genome sequences among viruses circulating in these bat populations, a level of diversity that would result from a long-term association with a suitable reservoir host of large population size.

Details

Language :
English
ISSN :
15537374 and 15537366
Volume :
5
Issue :
7
Database :
OpenAIRE
Journal :
PLoS Pathogens
Accession number :
edsair.doi.dedup.....fe7f698b1f003b883bdbdc697f81a33d