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Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors
- Source :
- Sensors, Volume 21, Issue 13, Sensors (Basel, Switzerland), Sensors, Vol 21, Iss 4348, p 4348 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Comparative analysis of the sensitivity of two surface plasmon resonance (SPR) biosensors was conducted on a single-metallic Au sensor and bimetallic Ag–Au sensor, using a cathepsin S sensor as an example. Numerically modeled resonance curves of Au and Ag–Au layers, with parameters verified by the results of experimental reflectance measurement of real-life systems, were used for the analysis of these sensors. Mutual relationships were determined between ∂Y/∂n components of sensitivity of the Y signal in the SPR measurement to change the refractive index n of the near-surface sensing layer and ∂n/∂c sensitivity of refractive index n to change the analyte’s concentration, c, for both types of sensors. Obtained results were related to experimentally determined calibration curves of both sensors. A characteristic feature arising from the comparison of calibration curves is the similar level of Au and Ag–Au biosensors’ sensitivity in the linear range, where the signal of the AgAu sensor is at a level several times greater. It was shown that the influence of sensing surface morphology on the ∂n/∂c sensitivity component had to be incorporated to explain the features of calibration curves of sensors. The shape of the sensory surface relief was proposed to increase the sensor sensitivity at low analyte concentrations.
- Subjects :
- Analyte
Materials science
Calibration curve
02 engineering and technology
TP1-1185
Biosensing Techniques
01 natural sciences
Biochemistry
Article
Analytical Chemistry
bimetallic biosensor
Sensitivity (control systems)
Electrical and Electronic Engineering
Surface plasmon resonance
Instrumentation
business.industry
Chemical technology
010401 analytical chemistry
Resonance
biomarkers
021001 nanoscience & nanotechnology
sensitivity
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Refractometry
Linear range
cathepsin determination
Optoelectronics
0210 nano-technology
business
Refractive index
Biosensor
surface plasmon resonance
Subjects
Details
- Language :
- English
- ISSN :
- 14248220
- Database :
- OpenAIRE
- Journal :
- Sensors
- Accession number :
- edsair.doi.dedup.....fe97fbd0612100fc4d370506c5974e3a
- Full Text :
- https://doi.org/10.3390/s21134348