Back to Search
Start Over
A new paper-based analytical device for detection of Glucose-6-phosphate dehydrogenase deficiency
- Source :
- Talanta. 164:534-539
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a genetic haemolytic disorder. Most persons with G6PD deficiency are asymptomatic, but exposure to oxidant drugs, such as the anti-malarial drug primaquine, may induce haemolysis, which is commonly found in Asian countries. A reliable test is necessary for diagnosing the deficiency to prevent an acute haemolytic crisis. This study proposes a novel quantitative method to detect G6PD deficiency using paper-based analytical devices (G6PDD-PAD). Wax printing was utilized for fabricating circular reaction zone patterns in paper. The colorimetric assay is based on the formation of formazan via a reduction of tetra-nitro blue tetrazolium (TNBT) by the G6PD enzyme on G6PDD-PAD. Detection was achieved by capturing the colour using a desktop scanner and the colour intensity was analysed with Adobe Photoshop C56. The results showed that the G6PD activity analysed by G6PDD-PAD was highly correlated with the standard biochemical assay (SBA) (r2=0.87, p
- Subjects :
- Paper
Primaquine
Dehydrogenase
02 engineering and technology
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
medicine
Humans
Detection limit
Chromatography
010401 analytical chemistry
Assay
Paper based
021001 nanoscience & nanotechnology
medicine.disease
Haemolysis
0104 chemical sciences
Glucosephosphate Dehydrogenase Deficiency
chemistry
Biochemistry
Printing
Colorimetry
Formazan
0210 nano-technology
Glucose-6-phosphate dehydrogenase deficiency
medicine.drug
Subjects
Details
- ISSN :
- 00399140
- Volume :
- 164
- Database :
- OpenAIRE
- Journal :
- Talanta
- Accession number :
- edsair.doi.dedup.....feb259bb0fc2b106562f6d1509f9b7c4