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Urease inhibitory constituents from Daphne retusa

Authors :
Itrat Anis
Muhammad Iqbal Choudhary
Ajmal Khan
Muhammad Raza Shah
Bishnu P. Marasini
Farrukh Mansoor
Source :
Journal of Asian Natural Products Research. 16:210-215
Publication Year :
2013
Publisher :
Informa UK Limited, 2013.

Abstract

The bioassay-guided fractionation of Daphne retusa Hemsl. has led to the isolation of a new aryl tetrahydronaphthalene lignan derivative named as daphnretusic acid (1), along with six new source compounds such as 5,7-dihydroxyflavone (2), 7-hydroxyflavone (3), 6-methoxyflavone (4), (+) pinoresinol (5), (+) sesamin (6), and β-sitosterol-3-O-β-D-glucopyranoside (7). Their structures were elucidated by (1)H NMR, (13)C NMR, 1D, 2D NMR, UV, IR, and EIMS analyses. All the fractions (n-hexane, CHCl3, AcOEt, CH3OH, and water) and pure compounds (1-7) were subjected to the assay of urease and α-chymotrypsin inhibitory activities. Chloroform and methanol soluble fractions showed moderate urease inhibition. Compound 2 exhibited significant urease inhibition with IC50 value 60.4 ± 0.72 μM, whereas compounds 1 and 3-7 remained inactive during urease inhibition and α-chymotrypsin bioassays.

Details

ISSN :
14772213 and 10286020
Volume :
16
Database :
OpenAIRE
Journal :
Journal of Asian Natural Products Research
Accession number :
edsair.doi.dedup.....fed7ca08eb3d569e760c1dbf7e55b5ec
Full Text :
https://doi.org/10.1080/10286020.2013.837457