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A nascent polypeptide sequence modulates DnaA translation elongation in response to nutrient availability
- Source :
- eLife, Vol 10 (2021), eLife
- Publication Year :
- 2021
- Publisher :
- eLife Sciences Publications Ltd, 2021.
-
Abstract
- The ability to regulate DNA replication initiation in response to changing nutrient conditions is an important feature of most cell types. In bacteria, DNA replication is triggered by the initiator protein DnaA, which has long been suggested to respond to nutritional changes, nevertheless the underlying mechanisms remain poorly understood. Here, we report a novel mechanism that adjusts DnaA synthesis in response to nutrient availability in Caulobacter crescentus. By performing a detailed biochemical and genetic analysis of the dnaA mRNA we identified a sequence downstream of the dnaA start codon that inhibits DnaA translation elongation upon carbon exhaustion. Our data show that the corresponding peptide sequence, but not the mRNA secondary structure or the codon choice, are critical for this response, suggesting that specific amino acids in the growing DnaA nascent chain tune translational efficiency. Our study provides new insights into DnaA regulation and highlights the importance of translation elongation as a regulatory target. We propose that nascent chain sequences, like the one described, might constitute a general strategy for modulating the synthesis rate of specific proteins under changing conditions.
- Subjects :
- genetic processes
Peptide Chain Elongation, Translational
translation
Start codon
Translational regulation
Caulobacter crescentus
Biology (General)
bacteria
Peptide sequence
0303 health sciences
Microbiology and Infectious Disease
biology
General Neuroscience
030302 biochemistry & molecular biology
Biochemistry and Molecular Biology
Translation (biology)
General Medicine
Chromosomes and Gene Expression
Cell biology
DNA-Binding Proteins
ribosome
Medicine
Research Article
DNA replication initiation
Translational efficiency
QH301-705.5
mRNA
Science
DNA replication
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Bacterial Proteins
RNA, Messenger
030304 developmental biology
Messenger RNA
General Immunology and Microbiology
030306 microbiology
E. coli
starvation
biology.organism_classification
DnaA
Culture Media
health occupations
Other
Peptides
Biokemi och molekylärbiologi
Subjects
Details
- Language :
- English
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....fed953848031f915601d6b6278e9ce77