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Differential expressions and functions of phosphodiesterase enzymes in different regions of the rat heart
- Source :
- European Journal of Pharmacology. 844:118-129
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Demirel-Yilmaz, Emine/0000-0002-2476-6225; Derici, Mehmet Kursat/0000-0002-8260-7492; Guray, Tulin/0000-0003-4572-1429 WOS: 000455010800015 PubMed: 30529467 Phosphodiesterase enzymes (PDEs) are responsible for the adjustment of cyclic nucleotide levels. Alterations in PDE expressions in different tissues cause conflicts between functional and clinical effects of PDE inhibitors. Therefore, the aim of this study was to investigate the gene and protein expressions and the functional role of PDEs in atrium and ventricle of rat heart The expressions of PDEs were examined in cardiac intact tissues and enzymatically isolated cells. The effects of PDE1-5 inhibitors (vinpocetine, EHNA, milrinone, rolipram, sildenafil, and IBMX) on basal and isoprenaline-stimulated contractions and sinus rate were recorded in the isolated spontaneously beating right atrium and electrically stimulated left papillary muscles. The mRNA and protein levels of PDEs were significantly different in atrial and ventricular intact tissues and isolated myocytes. Atrial contractions were increased with vinpocetine while suppressed by EHNA, milrinone, rolipram, sildenafil and IBMX. Milrinone, sildenafil and IBMX increased the heart rate whereas vinpocetine caused negative chronotropy. Papillary muscle contractions have been increased only with the vinpocetine and IBMX. Both the expression and the action of PDE-1-5 show atrial and ventricular differences. Therefore, these differences should be taken into account in the experimental or therapeutic approaches of the heart. Ankara University Research Foundation Ankara, TurkeyAnkara University [13B3330007] This study was supported by a research grants, 13B3330007 from the Ankara University Research Foundation Ankara, Turkey.
- Subjects :
- Male
0301 basic medicine
IBMX
Phosphodiesterase Inhibitors
Heart Ventricles
Expression
Pharmacology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Vinpocetine
Papillary muscle
medicine
Animals
Ventricular Function
Myocytes, Cardiac
Phosphodiesterase
Heart Atria
Rats, Wistar
Function
Rolipram
Right atrium
Phosphoric Diester Hydrolases
Papillary Muscles
Atrial Function
030104 developmental biology
medicine.anatomical_structure
chemistry
Ventricle
cardiovascular system
Milrinone
Female
EHNA
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- ISSN :
- 00142999
- Volume :
- 844
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmacology
- Accession number :
- edsair.doi.dedup.....fefc66e20724c43b1180d0c31355fbe4