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Sensitive and selective methods for the determination of cyproheptadine in tablets using N-bromosuccinimide and two dyes
- Source :
- Chemical Industry and Chemical Engineering Quarterly, Vol 18, Iss 3, Pp 449-458 (2012)
- Publication Year :
- 2012
- Publisher :
- Association of the Chemical Engineers of Serbia, 2012.
-
Abstract
- One titrimetric and two spectrophotometric methods are described for the determination of cyproheptadine hydrochloride (CPH) in bulk drug and tablets employing N-bromosuccinimide (NBS) as a brominating agent and two dyes, erioglaucine (EG) and meta-cresol purple (MCP) as auxiliary reagents. In titrimetry, a measured excess of NBS is added to an acidified solution of CPH and the unreacted NBS is determined iodometrically. Spectrophotometry involves the addition of a known excess of NBS to CPH in acid medium followed by estimation of residual NBS by reacting with a fixed amount of either erioglaucin and measuring the absorbance at 540 nm (method A) or meta-cresol purple and measuring the absorbance at 630 nm (method B). Titrimetric procedure is applicable over the range of 1.5-15 mg of CPH, and the reaction stoichiometry is found to be 1: 2 (CPH: NBS). The spectrophotometric methods are applicable over the ranges of 0.1-2.0 μg mL-1 (method A) and 0.4-12 μg mL-1 (method B). The molar absorptivities are calculated to be 1.4 × 105 and 2.2×104 Lmol-1cm-1 for method A and method B, respectively, and the corresponding Sandell sensitivity values are 0.0023 and 0.0141 μg cm-2. The limits of detection are calculated and found to be 0.03 and 0.24 μg mL-1 for method A and method B, respectively with corresponding limits of quantification 0.09 and 0.71. The methods were applied to the assay of CPH in tablets, and the results were compared statistically with those of a reference method.
- Subjects :
- Detection limit
N-bromosuccinimide
medicine.diagnostic_test
General Chemical Engineering
Analytical chemistry
Cyproheptadine
lcsh:TP155-156
titrimetry
pharmaceuticals
Cyproheptadine Hydrochloride
Absorbance
chemistry.chemical_compound
chemistry
Spectrophotometry
Reagent
medicine
spectrophotometry
Titration
N-Bromosuccinimide
lcsh:Chemical engineering
lcsh:HD9650-9663
Stoichiometry
Nuclear chemistry
lcsh:Chemical industries
Subjects
Details
- Language :
- English
- ISSN :
- 22177434 and 14519372
- Volume :
- 18
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Chemical Industry and Chemical Engineering Quarterly
- Accession number :
- edsair.doi.dedup.....ff0dd2a5d04868e99bd3280cac724839