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Microtubule disassembly by caspases is an important rate-limiting step of cell extrusion
- Source :
- Nature Communications, Nature Communications, 2022, 13 (1), pp.3632. ⟨10.1038/s41467-022-31266-8⟩
- Publication Year :
- 2022
- Publisher :
- HAL CCSD, 2022.
-
Abstract
- Epithelial cell death is essential for tissue homeostasis, robustness and morphogenesis. The expulsion of epithelial cells following caspase activation requires well-orchestrated remodeling steps leading to cell elimination without impairing tissue sealing. While numerous studies have provided insight about the process of cell extrusion, we still know very little about the relationship between caspase activation and the remodeling steps of cell extrusion. Moreover, most studies of cell extrusion focused on the regulation of actomyosin and steps leading to the formation of a supracellular contractile ring. However, the contribution of other cellular factors to cell extrusion has been poorly explored. Using the Drosophila pupal notum, a single layer epithelium where most extrusion events are caspase-dependent, we first showed that the initiation of cell extrusion and apical constriction are surprisingly not associated with the modulation of actomyosin concentration/dynamics. Instead, cell apical constriction is initiated by the disassembly of a medio-apical mesh of microtubules which is driven by effector caspases. We confirmed that local and rapid increase/decrease of microtubules is sufficient to respectively expand/constrict cell apical area. Importantly, the depletion of microtubules is sufficient to bypass the requirement of caspases for cell extrusion. This study shows that microtubules disassembly by caspases is a key rate-limiting steps of extrusion, and outlines a more general function of microtubules in epithelial cell shape stabilisation.<br />This a pre-release prior to the publication of the article. It will be later updated
- Subjects :
- Multidisciplinary
[SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE]
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
apoptosis
morphogenesis
General Physics and Astronomy
[SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis
Actomyosin
General Chemistry
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy
Microtubules
[INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation
Epithelium
General Biochemistry, Genetics and Molecular Biology
developmental biology
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
cell death
Caspases
cell extrusion
Animals
Drosophila
[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Database :
- OpenAIRE
- Journal :
- Nature Communications, Nature Communications, 2022, 13 (1), pp.3632. ⟨10.1038/s41467-022-31266-8⟩
- Accession number :
- edsair.doi.dedup.....ff4c99e4f92bb0d24d313a25558331e8
- Full Text :
- https://doi.org/10.1038/s41467-022-31266-8⟩