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MicroRNA-101 is a potential prognostic indicator of laryngeal squamous cell carcinoma and modulates CDK8
- Source :
- Journal of Translational Medicine
- Publication Year :
- 2015
- Publisher :
- BioMed Central, 2015.
-
Abstract
- Background Various microRNAs (miRNAs) negatively modulate genes that are involved in cellular proliferation, differentiation, invasion, and apoptosis. In many types of cancer, the expression profiles of these miRNAs are altered. Recently, miR-101 was identified as a tumour suppressor and was found to be expressed at low levels in various types of tumours, including prostate, breast, endometrium, and bladder cancers. However, the function(s) of miR-101 in laryngeal carcinoma remain unknown. Methods The expression levels of miR-101 in laryngeal squamous cell carcinoma (LSCC) tissues and cells were detected by qPCR. Cell proliferation, migration, cell cycle, and apoptosis assay were applied to assess the function(s) of miR-101 in vitro. Nude mice subcutaneous tumour model was used to perform in vivo study. Moreover, we identified Cyclin-dependent kinase 8 (CDK8) as the target of miR-101 by a luciferase assay. The possible downstream effectors of CDK8 were investigated in Wnt/β-catenin signaling pathway. Changes of CDK8, β-catenin, and cyclin D1 protein levels were analyzed by western blotting and immunohistochemical staining. The prognostic effect of miR-101 was evaluated using the Kaplan–Meier method. Results Expression of miR-101 was down-regulated in the LSCC tissues compared with the adjacent normal tissues. Furthermore, downregulation of miR-101 correlated with T3–4 tumour grade, lymph node metastasis, and an advanced clinical stage in the LSCC patients examined (P
- Subjects :
- Male
MiR-101
CDK8
Proliferation
Apoptosis
Laryngeal squamous cell carcinoma
Biology
Polymerase Chain Reaction
General Biochemistry, Genetics and Molecular Biology
Cell Line
Mice
Cyclin D1
Cell Movement
Cell Line, Tumor
microRNA
Carcinoma
medicine
Animals
Humans
Genes, Tumor Suppressor
Neoplasm Invasiveness
Laryngeal Neoplasms
Wnt Signaling Pathway
beta Catenin
Cell Proliferation
Medicine(all)
Mice, Inbred BALB C
Cell growth
Biochemistry, Genetics and Molecular Biology(all)
Research
Gene Expression Profiling
Cell Cycle
Wnt signaling pathway
Cancer
General Medicine
Cell cycle
medicine.disease
Cyclin-Dependent Kinase 8
Molecular biology
Xenograft Model Antitumor Assays
Gene expression profiling
Gene Expression Regulation, Neoplastic
MicroRNAs
Carcinoma, Squamous Cell
Subjects
Details
- Language :
- English
- ISSN :
- 14795876
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Translational Medicine
- Accession number :
- edsair.doi.dedup.....ff644c79c710717b2611b9a821913600