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Characterization of proteases activities in Ficus carica cultivars
- Source :
- Journal of food scienceREFERENCES. 85(3)
- Publication Year :
- 2019
-
Abstract
- In this study, we characterized protease activities of 23 Ficus carica cultivars. Extracts of fruit, branch, and leaf of Masui Dauphine, one of the most representative F. carica cultivars in Japan, exhibited gelatin-hydrolyzing activity, both in the absence and presence of a cysteine protease-specific inhibitor, E-64, suggesting that not only ficin (classified as cysteine protease) but also collagenase (classified as serine protease) were involved in the digestion of gelatin. In the hydrolysis of (7-methoxycoumarin-4-yl)acetyl-l-Lys-l-Pro-l-Leu-Gly-l-Leu-[N3 -(2,4-dinitrophenyl)-l-2,3-diaminopropionyl]-l-Ala-l-Arg-NH2 , all branch extracts of 23 F. carica cultivars exhibited the activity both in the absence and presence of cysteine protease-specific inhibitor E-64, indicating that they contain ficin and collagenase. During digestion of acid-solubilized type I collagen by the branch extract of Masui Dauphine at 40-55 °C, collagen was completely digested in the absence of E-64, while it was partially digested in the presence of the inhibitor, indicating that the manner of digestion differed between ficin and collagenase contained in the extract. These results suggest that F. carica is attractive for industrial use to digest collagen. PRACTICAL APPLICATION: The industrial use of F. carica might be enhanced by efficiently utilizing these proteases and/or selecting the appropriate F. carica cultivar. Collagen is one of the targets to which our results might be applied. It is widely accepted today that collagen and its digestion products could be useful as functional food. F. carica is a potential candidate for use in not only complete but also partial digestion of collagen.
- Subjects :
- Proteases
030309 nutrition & dietetics
medicine.medical_treatment
E-64
03 medical and health sciences
chemistry.chemical_compound
0404 agricultural biotechnology
Japan
medicine
Plant Proteins
Serine protease
0303 health sciences
Protease
biology
04 agricultural and veterinary sciences
biology.organism_classification
Ficus
040401 food science
Cysteine protease
Plant Leaves
Biochemistry
chemistry
Fruit
Proteolysis
biology.protein
Collagenase
Biocatalysis
Collagen
Carica
Type I collagen
Food Science
medicine.drug
Peptide Hydrolases
Subjects
Details
- ISSN :
- 17503841
- Volume :
- 85
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of food scienceREFERENCES
- Accession number :
- edsair.doi.dedup.....ff6769a897f4485cf0762afcc38eea65