Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo

Procedure of manufacturingK. pinnatawater extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract ofK. pinnata+ CecP1 exhibits favorable effect on healing of wounds infected withS. aureus(equal to Cefazolin) and with a combination ofS. aureuswithP. aeruginosa(better than Cefazolin). Wild-typeK. pinnataextract exhibited evident microbicide activity againstS. aureuswithP. aeruginosabut it was substantially strengthened inK. pinnata+ CecP1 extract.K. pinnataextracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to immunomodulating activity, wild-typeK. pinnataextract accelerated granulation of the wound bed and marginal epithelialization even better thanK. pinnata+ CecP1 extract. Immunomodulating and microbicide activity ofK. pinnatasynergizes with microbicide activity of CecP1 accelerating elimination of bacteria.