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RNA degradation as described by a mathematical model for postmortem interval determination
- Source :
- Journal of Forensic and Legal Medicine. 44:43-52
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Precisely determining the postmortem interval (PMI) is crucial to civil, criminal and forensic cases. A technique to exploit the postmortem RNA transcript level was developed to increase the accuracy and practicality of PMI estimation. For this purpose, lung tissues and muscle tissues were removed at twelve time points (0–144 h) from rat corpses that had been stored at three different temperatures (10, 20 and 30 °C). Human tissues were collected at autopsy from twelve real cases with known PMI values and other parameters. After the RNA was extracted from all these samples, the transcript levels of nine biomarkers were analyzed by real-time quantitative PCR (RT-qPCR). With the assistance of geNorm, miR-195 , miR-200c , 5S , U6 and RPS29 were selected as reference biomarkers for lung specimens; miR-1 , miR-206 , 5S and RPS29 were chosen as control markers for muscle tissues. On the contrary, ACTB and GAPDH were significantly correlated with the PMI. The mathematical models using these target biomarkers were constructed to describe the characteristic relationship between △Ct values (normalized to reference biomarkers) and the observed PMI for each temperature group. Following validation, the relatively low error rates (7.4% and 12.5% for rat and human samples, respectively) demonstrated the accuracy and reliability of the mathematical model. We believe these results indicate that the multi-parametric mathematical model can become a practical tool for PMI estimation.
- Subjects :
- Adult
Male
Ribosomal Proteins
0301 basic medicine
Pathology
medicine.medical_specialty
Adolescent
RNA Stability
Interval (mathematics)
Biology
Transcript level
Real-Time Polymerase Chain Reaction
Pathology and Forensic Medicine
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
030216 legal & forensic medicine
Muscle, Skeletal
Lung
Models, Statistical
General Medicine
Rna degradation
Middle Aged
Actins
MicroRNAs
Pmi estimation
030104 developmental biology
RNA, Ribosomal
Child, Preschool
Postmortem Changes
Female
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
Law
Subjects
Details
- ISSN :
- 1752928X
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Journal of Forensic and Legal Medicine
- Accession number :
- edsair.doi.dedup.....ffa981ec2faac60bedba30becffa9b87