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Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus
- Source :
- Frontiers in Immunology, Frontiers in Immunology, Vol 12 (2021)
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- In cystic fibrosis (CF) infectious and allergic airway inflammation cause pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common long-term colonizer and cause of recurrent airway infections in CF. The pathogen is also associated with respiratory allergy; especially the staphylococcal serine protease-like proteins (Spls) can induce type 2 immune responses in humans and mice. We measured the serum IgE levels specific to 7 proteases of S. aureus by ELISA, targeting 5 Spls (76 CF patients and 46 controls) and the staphopains A and B (16 CF patients and 46 controls). Then we compared cytokine release and phenotype of T cells that had been stimulated with Spls between 5 CF patients and 5 controls. CF patients had strongly increased serum IgE binding to all Spls but not to the staphopains. Compared to healthy controls, their Spl-stimulated T cells released more type 2 cytokines (IL-4, IL-5, IL-13) and more IL-6 with no difference in the secretion of type 1- or type 3 cytokines (IFNγ, IL-17A, IL-17F). IL-10 production was low in CF T cells. The phenotype of the Spl-exposed T cells shifted towards a Th2 or Th17 profile in CF but to a Th1 profile in controls. Sensitization to S. aureus Spls is common in CF. This discovery could explain episodes of allergic inflammation of hitherto unknown causation in CF and extend the diagnostic and therapeutic portfolio.
- Subjects :
- Male
0301 basic medicine
Allergy
T-Lymphocytes
medicine.medical_treatment
Immunoglobulin E
medicine.disease_cause
Cystic fibrosis
Type 2 immune response
cystic fibrosis
0302 clinical medicine
Immunology and Allergy
Cells, Cultured
Original Research
type 2 immune response
biology
Staphylococcal Infections
Antibodies, Bacterial
Healthy Volunteers
Cytokine
Staphylococcus aureus
Host-Pathogen Interactions
Female
IgE
lcsh:Immunologic diseases. Allergy
Adolescent
Primary Cell Culture
Immunology
Allergic inflammation
Young Adult
03 medical and health sciences
Immune system
Bacterial Proteins
Hypersensitivity
medicine
Animals
Humans
business.industry
allergy
medicine.disease
Th2 cells
030104 developmental biology
030228 respiratory system
Case-Control Studies
biology.protein
Serine Proteases
lcsh:RC581-607
business
Subjects
Details
- ISSN :
- 16643224
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology
- Accession number :
- edsair.doi.dedup.....ffc453c1a899632fce100affc5c1a914
- Full Text :
- https://doi.org/10.3389/fimmu.2021.651060