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Guava flavonoid glycosides prevent influenza A virus infection via rescue of P53 activity
- Source :
- Journal of Medical Virology. 91:45-55
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Influenza is a highly infectious disease caused by three types of viruses, including influenza A virus (IAV), influenza B virus, and, rarely, influenza C virus. IAV is a major, global public health threat, causing approximately 500 000 deaths per year worldwide. The new strains of IAV have emerged due to a mutation called antigenic shift, which results in a new subtype of the virus that shows resistance to common antiviral drugs. Here, guava and lemon extracts, including green leaves and flowers, were investigated for their activity against IAV replication in human A549 cells. Concomitantly, the cytotoxicity of a potent extract on host-cell multiplication was assessed. Our results reveal that guava extracts inhibit IAV replication, indicated by viral nucleoprotein expression profile and traditional plaque assay. Interestingly, treatment with guava extract inactivates Akt protein kinase and stimulates the pro-apoptotic protein P53, at early stages of infection. Furthermore, purified guava flavonoid glycosides (GFGs) show competitive inhibition of IAV-virus replication via early regulation of IL-1β and IL-8 in association with P53 gene expression. The docking analysis of GFGs and the protein structure of upstream targets for the Akt signaling pathway indicates a sufficient interaction and stabilization with Gbr2 protein. These data indicate that treatment with GFGs disturbs IAV replication via activation of P53 and its apoptotic related factors after infection. Collectively, these data show that targeting of essential host kinases that are involved in the replication cycle of IAV and rescue of P53 activity by GFGs could represent a new strategy to eradicate IAV.
- Subjects :
- Citrus
Interleukin-1beta
Viral Plaque Assay
Biology
Virus Replication
medicine.disease_cause
Antiviral Agents
Virus
03 medical and health sciences
0302 clinical medicine
Virology
Influenza A virus
medicine
Humans
Glycosides
030212 general & internal medicine
Protein kinase B
Virus quantification
Psidium
Plant Extracts
Akt/PKB signaling pathway
Kinase
Viral Core Proteins
Interleukin-8
RNA-Binding Proteins
Antigenic shift
Nucleocapsid Proteins
Infectious Diseases
A549 Cells
030211 gastroenterology & hepatology
Tumor Suppressor Protein p53
Influenza C Virus
Subjects
Details
- ISSN :
- 10969071 and 01466615
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Virology
- Accession number :
- edsair.doi.dedup.....ffd2511feb48858739b5f44023cb9313
- Full Text :
- https://doi.org/10.1002/jmv.25295