BACTERIAL EXPRESSION OPTIMISATION OF ANTITUMOR CYTOKINE EMAP II IN ESCHERICHIA COLI BL21(DE3) PLYSE CELLS

EMAP II (Endothelial Monocyte-Activating Polypeptide) – a new antiangiogenic proinflammatory cytokine that exhibits antitumor activity. In order to develop genetically engineered technology for EMAP II optimization of bacterial expression conditions within pET30a vector encoded EMAP II was carried out. Both the influence of target protein synthesis inductor IPTG concentration on its overall yield and optimal bacterial cultivation time before and after inductor addition were estimated. There was a proposed scheme for the cultivation of culture E. colі BL21(DE3)pLysE to achieve high yield of recombinant cytokine EMAP II at the level of 110 mg from 1 liter of bacterial culture.