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Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential

Authors :
Dautu, George
Munyaka, Biscah
Carmen, Gabriella
Zhang, Guohong
Omata, Yoshitaka
Xuan, Xuenan
Igarashi, Makoto
Source :
Experimental Parasitology. 116(3):273-282
Publication Year :
2007
Publisher :
Elsevier, 2007.

Abstract

application/pdf<br />A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-γ synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific immune response and also provides effective protection against toxoplasmosis more than the MIC2, M2AP and BAG1 genes.

Details

Language :
English
ISSN :
00144894
Volume :
116
Issue :
3
Database :
OpenAIRE
Journal :
Experimental Parasitology
Accession number :
edsair.jairo.........3f14d68c2b96a9da36f165d94f2479c9