Ketone body metabolism in muscle (III) : Changes in keton body concentration in the 12 Hz-stimulated muscle

Physical training has been shown to improve insulin action in simple obese and obese diabetic patients. On the other hand,the hyperketonemic effect of prolonged exercise in insulin-dependent diabetic patients is well recognized phenomenon. Recently, we have demonstrated that 60-min high-frequency (60Hz) stimulation produces a significant reduction in 3-hydroxybutyrate (3-OHBA) uptake in muscle not only in diabetic but also in control rats and that a reduction in 3-OHBA utilization in diabetics is found for 90 min after muscle constriction. In the present study. we measured extracellular 3-OHBA concentrations in 12Hz-stimulated muscle for five diabetic and five control rats by use of the microdialysis technique. The following results were obtained: 1. In control rats, there was no significant difference in 3-OHBA levels between before, during and after stimulation. 2. In diabetics, the dialysate 3-OHBA concentrations showed a significant increase during constriction and for 90 min after constriction. It is well known that 12Hz-stimuli is more aerobic in muscle than 60-Hz-stimuli. Therefore, it may be advisable that diabetic patients performe an aerobic exercise, in order to increase insulin action in peripheral tissue, after nearly normalization for metabolism by diet and insulin therapy, and so on.