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Conserved Roles of Fibroblast Growth Factor Receptor 2 Signaling in the Regulation of Inner Cell Mass Development in Bovine Blastocysts
- Source :
- Molecular reproduction and development. 83(6):516-525
- Publication Year :
- 2016
- Publisher :
- Wiley-Blackwell, 2016.
-
Abstract
- A common process during preimplantation mammalian development is blastocyst formation, which utilizes signaling through fibroblast growth factor receptor 2 (FGFR2), yet the mechanisms through which FGFR2 signaling affect preimplantation development in bovine embryos remain incompletely understood. Here, we used RNA-interference to investigate the in vitro development, the frequency of blastomere apoptosis, and the mRNA expression of developmental marker genes in FGF receptor 2-knockdown (FGFR2-KD) bovine embryos. A reduction in FGFR2 mRNA did not affect preimplantation development or the frequency of apoptotic blastomeres, but did enhanced proliferation of the inner cell mass in blastocysts (P< 0.05) -which differs from the phenotype reported for bovine embryos using a pharmacological approach (treatment with the pan-FGFR blocker PD173074), but agrees with previous results obtained using mouse embryos. Moreover, the expression of an epiblast marker gene, NANOG, and a primitive endoderm marker gene, GATA6, remained unchanged, whereas the expression of another primitive endoderm marker gene, HNF4A, was significantly reduced in FGFR2-KD embryos. Therefore, FGFR2 signaling appears to be associated with the regulation of inner cell mass development and proliferation during blastocyst formation in cattle.
Details
- Language :
- English
- ISSN :
- 1040452X
- Volume :
- 83
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecular reproduction and development
- Accession number :
- edsair.jairo.........458da57a2c9ad5f354bd74dd01464074