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Fluorescent in situ hybridization to ascidian chromosomes

Authors :
Shoguchi, Eiichi
Ikuta, Tetsuro
Yoshizaki, Fumiko
Satou, Yutaka
Satoh, Nori
Asano, Katsutoshi
Saiga, Hidetoshi
Nishikata, Takahito
Source :
Zoological Science. 21(2):153-157
Publication Year :
2004
Publisher :
Zoological Society of Japan, 2004.

Abstract

The draft genome of the ascidian Ciona intestinalis has been sequenced. Mapping of the genome sequence to the Ciona 14 haploid chromosomes is essential for future studies of the genome-wide control of gene expression in this basal chordate. Here we describe an efficient protocol for fluorescent in situ hybridization for mapping genes to the Ciona chromosomes. We demonstrate how the locations of two BAC clones can be mapped relative to each other. We also show that this method is efficient for coupling two so-far independent scaffolds into one longer scaffold when two BAC clones represent sequences located at either end of the two scaffolds.

Details

Language :
English
ISSN :
02890003
Volume :
21
Issue :
2
Database :
OpenAIRE
Journal :
Zoological Science
Accession number :
edsair.jairo.........a3ee6249f219dd3878a62d24f98d61fa