Effects of the CDT6/ANG X gene on tumour growth in immune competent mice

Background: Cornea-derived transcript 6 (CDT6, also known as AngX) has been described to inhibit tumour growth in a human melanoma growing in nude mice. In another report, however. enhancement of tumour growth in comparable circumstances occurred. We therefore studied the effect of the same gene in different circumstances, using an immune competent mouse model. Materials and Methods: In this report we describe the generation of a stably CDT6-expressing clone of the murine melanoma cell line B-16-F10. These cells were implanted in female C57/B16 mice with empty vector transfected cells as control. Results: We found no significant inhibition or stimulation of either lag-time or doubling-time of the tumours. In addition no effect of the CDT6 gene product was found on proliferation of endothelial cells in vitro. Conclusion: In contrast to air immune deficient mouse model, no anti-tumour effect could be detected with the CDT6 gene product in an immune competent mouse model.