Mapping of enzymatic kinetics on a chip

We show the development of a microfluidic platform that is capable of performing 36 parallel reactions with 36 combinations of the concentrations of two reagents. With the device, we obtained a 3D enzymatic reaction plot of horseradish peroxidase (HRP) with Amplex Red (AR) and hydrogen peroxide (H2O2) in a nanoliter scale volume. The system could be used as a fast analytical tool to evaluate various chemical and biochemical reactions especially where two or more reagents interact with each other.