Identification genetic characterization and population of Barbus brachycephalic caspius, Lucioperca lucioperca, Rutilus rutilus caspius, Rutilus frisii kutum and Salmo trutta caspius in southern part of the Caspian Sea by molecular method (Microsatellites) and formation DNA bank

In this study genetic characterization of Barbus brachycephalus caspius, Lucioperca lucioperca, Rutilus rutilus caspius, Rutilus frisi kutum and Salmo trutta caspius were examined by 611 samples from regions in East (Guilan province), Middle (Mazandaran province) and west (Golestan province) of southen part of the Caspian Sea. DNA was extracted from fin tissue by phenol -chlorophorm method and then PCR was performed using special primers. Statistical analysis of data was performed by Gene Alex, MEGA and Arlequin softwares. -Rutilus frisi kutum: The results showed that nine of ten primers were polymorphic loci. The mean of effective and observed alleles were 7.26±0.49 and 4.37±0.35 respectively. Also, the mean of expected and observed heterozygosity were 0.55±0.03 and 0.69±0.02 respectively Of the analysed loci, all of the samples (except Tajan and samples in LOC4 and Gilan samples in MFW2) possible tests were found to deviate significantly from the Hardy–Weinberg equilibrium (P