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NK and CD8+ T cell phenotypes predict onset and control of CMV viremia after kidney transplant
- Source :
- JCI insight, vol 6, iss 21
- Publication Year :
- 2021
- Publisher :
- eScholarship, University of California, 2021.
-
Abstract
- CMV causes mostly asymptomatic but lifelong infection. Primary infection or reactivation in immunocompromised individuals can be life-threatening. CMV viremia often occurs in solid organ transplant recipients and associates with decreased graft survival and higher mortality. Furthering understanding of impaired immunity that allows CMV reactivation is critical to guiding antiviral therapy and examining the effect of CMV on solid organ transplant outcomes. This study characterized longitudinal immune responses to CMV in 31 kidney transplant recipients with CMV viremia and matched, nonviremic recipients. Recipients were sampled 3 and 12 months after transplant, with additional samples 1 week and 1 month after viremia. PBMCs were stained for NK and T cell markers. PBMC transcriptomes were characterized by RNA-Seq. Plasma proteins were quantified by Luminex. CD8+ T cell transcriptomes were characterized by single-cell RNA-Seq. Before viremia, patients had high levels of IL-15 with concurrent expansion of immature CD56bright NK cells. After viremia, mature CD56dim NK cells and CD28-CD8+ T cells upregulating inhibitory and NK-associated receptors were expanded. Memory NK cells and NK-like CD28-CD8+ T cells were associated with control of viremia. These findings suggest that signatures of innate activation may be prognostic for CMV reactivation after transplant, while CD8+ T cell functionality is critical for effective control of CMV.
- Subjects :
- Adult
Male
Kidney Disease
Immunology
T cells
CMV Systems Immunobiology Group
NK cells
CD8-Positive T-Lymphocytes
Vaccine Related
Young Adult
Clinical Research
Biodefense
Killer Cells
Humans
Viremia
Aged
Transplantation
Organ transplantation
Prevention
virus diseases
Middle Aged
Kidney Transplantation
Phenotype
Infectious Diseases
Good Health and Well Being
Cytomegalovirus Infections
Natural
Female
Infection
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- JCI insight, vol 6, iss 21
- Accession number :
- edsair.od.......325..979e84621be9aefe4167a087c12b1e41