Kromatografska identifikacija serotonina u koprivi Urtica pilulifera var. dodartii (L.) Aschers

Leaves and stems of the Urtica pilulifera var. dodartii were homogenized in cold 80% methanol and extracted several times during 48 hours. The separation of the compound was achieved on paper chromatography and an Amberlite CG-50 column. The identification was based on comparisons of Rf values and colour reactions with the authentic sample of serotonin.