Tumorska transformacija u tkivu gomolja krumpira

Potato (Solarium tuberosum L. cv. Desirée) tuber tissue was infected with Agrobacterium tumefaciens, strains: B6S3 (wild type), pGV2255 (rooty mutant), pGV2215 (shooty mutant) and with Agrobacterium rhizogenes (8196). Successful tumor induction was achieved by B6S3 (unorganised tumors) and by pGV2255 (rooty tumors). Transformed tuber cells were smaller than normal ones and free from starch. Transformation process caused a significant increase in peroxidase activity which can be taken as a tumorigenetic marker. Electrophoretic protein pattern showed that tumor cells stopped producing a polypeptide of about 40 kd, which was a storage protein dominant in normal tissue.
Tkivo gomolja krumpira inficirale smo bakterijom Agrobacterium tumefaciens (sojevi: B6S3, pGV2215, pGV2255) i A. rhizogenes (8196). Uspješnu tumorsku transformaciju postigle smo divljim sojem (B6S3) i mutiranim (pGV2255) koji izaziva tumore s korijenom. Svjetlosnim mikroskopom mogle smo razlikovati normalne stanice od tumorskih. Tumorska transformacija odrazila se u porastu peroksidazne aktivnosti te stoga, ovaj enzim može poslužiti kao pokazatelj transformacijskih procesa. Elektroforetskim razdvajanjem topivih proteina u SDS-poliakrilamidnom gelu utvrdile smo da tumorske stanice prestaju sintetizirati proteinsku prugu na položaju koji odgovara molekularnoj težini od oko 40 kd.