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Agrobacterium tumefaciens Deploys a Superfamily of Type VI Secretion DNase Effectors as Weapons for Interbacterial Competition In Planta
- Publication Year :
- 2014
- Publisher :
- Elsevier, 2014.
-
Abstract
- The type VI secretion system (T6SS) is a widespread molecular weapon deployed by many Proteobacteria to target effectors/toxins into both eukaryotic and prokaryotic cells. We report that Agrobacterium tumefaciens, a soil bacterium that triggers tumorigenesis in plants, produces a family of type VI DNase effectors (Tde) that are distinct from previously known polymorphic toxins and nucleases. Tde exhibits an antibacterial DNase activity that relies on a conserved HxxD motif and can be counteracted by a cognate immunity protein, Tdi. In vitro, A. tumefaciens T6SS could kill Escherichia coli but triggered a lethal counterattack by Pseudomonas aeruginosa upon injection of the Tde toxins. However, in an in planta coinfection assay, A. tumefaciens used Tde effectors to attack both siblings cells and P. aeruginosa to ultimately gain a competitive advantage. Such acquired T6SS-dependent fitness in vivo and conservation of Tde-Tdi couples in bacteria highlights a widespread antibacterial weapon beneficial for niche colonization.
- Subjects :
- BIOFILMS
Virulence Factors
Immunology
VIBRIO-CHOLERAE
IMMUNITY
Microbiology
1108 Medical Microbiology
Virology
Antibiosis
Tobacco
Escherichia coli
Bacterial Secretion Systems
Science & Technology
Deoxyribonucleases
IDENTIFICATION
GENE
Anti-Bacterial Agents
ICMF FAMILY PROTEIN
Agrobacterium tumefaciens
Pseudomonas aeruginosa
TARGET-CELLS
Parasitology
TN-SEQ
PSEUDOMONAS
Life Sciences & Biomedicine
SYSTEM
0605 Microbiology
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.od......1032..970d00dc5f3c86a06c1cb9907040f608