Paraffin immunofluorescence, an ancillary technique in renal pathology - one center study

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department: Department of Biological and Medical Sciences Study program: Bioanalytical laboratory diagnostics in healthcare Author: Bc. Karolína Buchová Supervisior: RNDr. Ivana Němečková, Ph.D. Consultant: doc. MUDr. Kristýna Pivovarčíková, Ph.D. Title of Thesis: Paraffin immunofluorescence, an ancillary technique in renal pathology - one center study Background: Immunofluorescence analysis (IFLU) is routinely used to diagnose non-tumor renal diseases, and also has a place in renal transplant pathology. This examination is most often performed on frozen sections from non-fixed tissue. The next option where we could use the IFLU method is examination in formaldehyde-fixed tissues which are embedded in paraffin blocks. However, using the formaldehyde-fixed paraffin-embeded samples has its specifics and require a modification of the methodology and verification. This diploma thesis follows the differences in the processing of renal punctures when we use IFLU for frozen material (FIF) or IFLU for formalin-fixed and paraffin- embedded tissue (PIF) and comparison of the results obtained by both methods. Main findings: Immunofluorescence examination is very important part in examination of kidney biopsy obtained by puncture. The...