Effect of Anadenanthera colubrina extract on the healing of excisional skin wound induced in mice

Introduction: Wound healing is a complex skin repair process that can be impaired due to exacerbated inflammation, causing consequences physical, psychological and economic. In this sense, medicinal plants are promising alternatives in wound repair, mainly due to the modulation of inflammation. Anadenanthera colubrina, popularly known as angico, has been widely used in traditional medicine and has important medicinal properties anti-inflammatory, antioxidant, antimicrobial and healing. However, studies on skin repair remain scarce and scarring mechanisms are little explored. Objective: To evaluate the effect of the Ethanol Extract of A. colubrina (EEAc) on the healing of excision cutaneous wounds induced in mice. Methodology: Swiss female mice were submitted to dorsothoracic cutaneous excision with a 6 mm metallic punch and distributed in a control group, vehicle or EEAc 5%. The wound area was measured on days 0, 3, 5, 7, 10 and 14 and the tissues collected on days 1, 3, 7 and/or 14 to determine the activity of Myeloperoxidase (MPO), quantification of N-acetyl- β-D-glycosaminidase (NAG), measurement of TNF-α and IL-10 and analysis of leukocyte infiltrate, angiogenesis, re-epithelialization and collagen deposition in lesions. Results: The EEAc 5% induced a reduction (p