Healing hydrogel based in natural hemicelluloses from Caesalpinea pulcherrima and laticifers proteins of Calotropis procera

VASCONCELOS, Mirele da Silveira. Hidrogel cicatrizante a partir de hemiceluloses vegetais de Caesalpinea pulcherrima e proteínas laticíferas de Calotropis procera. 2017.139 f.Tese (Doutorado em Bioquímica)-Universidade Federal do Ceará, Fortaleza, 2017. Calotropis procera (Ait.) R. Br. is well known for being a latcifers plant and for its medicinal properties. Its latex has already been used in traditional medicine in the treatment of dermis disorders. Scientific studies have shown that laticifers proteins isolated from C. procera have several pharmacological properties and their anti-inflammatory, antiseptic, analgesic and wound healing effects are reported. The aim of this work was to evaluate the effect of the CpPII protein fraction of C. procera on cicatricial process modulation in experimental wound models. For this, a hydrogel (Hg) was developed using natural hemicelluloses (galactomannans) from Caesalpinea pulcherrima, as excipient, and laticifers proteins (CpPII), rich in proteolytic enzymes as active principle. In the present study, laticifers proteins containing proteolytic activity (CpPII) or inhibited with iodoacetamide (IAA) were incorporated at different concentrations (w/v) in galactomannan hydrogels (Hemi) to obtain the following formulations tested: HgCpPII 0.2%, HgCpPII 0.5% and HgCpPII-IAA 0.2%. Male Swiss mice were used for in vivo tests (wound healing and dermal irritability) according to experimental procedures approved by the institutional ethics committee under number 64/2014. Tissue modifications and target mediators of cicatricial response were evaluated according to morphometric, biochemical, immunological and histological parameters in excisional wound models (1cm2). The results showed that the hydrogel formulations presented satisfactory quality after microbiological analysis, storage stability at 4ºC and dermal biocompatibility. Wounds treated with the CpPII fraction showed modulation of inflammatory mediators (MPO, NO, IL-1β, IL-6) and reduction of lipid peroxidation (MDA). The number of mast cells degranulated in the CpPII-treated animals increased significantly on day 14, and the mast cells degranulation in the group treated with 0.5% HgCpPII (p