Utiliza??o de marcadores SSR (Simple Sequence Repeats) na an?lise da diversidade gen?tica de matrizes de Pinus taeda Linnaeus

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2019-02-01T18:25:58Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) viviane_ferreira_anjos.pdf: 955893 bytes, checksum: 59ddc9cb28b9b3d67f2088979c39eb45 (MD5) Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2019-03-07T16:49:00Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) viviane_ferreira_anjos.pdf: 955893 bytes, checksum: 59ddc9cb28b9b3d67f2088979c39eb45 (MD5) Made available in DSpace on 2019-03-07T16:49:00Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) viviane_ferreira_anjos.pdf: 955893 bytes, checksum: 59ddc9cb28b9b3d67f2088979c39eb45 (MD5) Previous issue date: 2018 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) Ap?s sua introdu??o em 1948, o cultivo de Pinus taeda L. ocorre nas regi?es sul e sudeste do Brasil e tem por finalidade, basicamente, abastecer os setores de celulose de fibras longas, pain?is reconstitu?dos, constru??o civil, ind?stria moveleira e servir como fonte de energia (lenha industrial). Diante da sua import?ncia econ?mica, pesquisadores buscam ferramentas que auxiliem no aumento da qualidade e da produtividade dos plantios e com isso, passaram a fazer uso dos marcadores moleculares, a fim de obter informa??es a respeito principalmente da diversidade gen?tica entre os indiv?duos que comp?em a popula??o de sele??o. Assim, com vistas a contribuir com o programa de melhoramento do Pinus taeda, utilizou-se marcadores moleculares microssat?lites (SSR) para detectar a exist?ncia de polimorfismos entre 124 plantas de um pomar de sementes. Para o desenvolvimento do estudo, foi realizada a extra??o do DNA gen?mico total do material vegetal (ac?culas) das 124 plantas de Pinus taeda, que ap?s esse processo, foram amplificados por 5 pares de iniciadores microssat?lites espec?ficos para a esp?cie. Os produtos da PCR foram separados em gel de poliacrilamida n?o desnaturante a 6%, corado com nitrato de prata e analisados visualmente, e em gel de agarose a 3%, corado com Brometo de Et?deo. Os marcadores moleculares microssat?lites utilizados permitiram analisar a diversidade gen?tica das plantas de Pinus taeda avaliadas. As informa??es de diversidade obtidas poder?o auxiliar no processo de melhoramento gen?tico da esp?cie. A depender do objetivo do programa de melhoramento, a popula??o e sua diversidade poder?o ser manipuladas, mediante cruzamentos e sele??es devidamente direcionados. Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2018. After its introduction in 1948, the cultivation of Pinus taeda L. occurs in the southern and southeastern regions of Brazil and its main purpose is to supply the long fiber cellulose, reconstituted panels, civil construction, furniture industry and serve as sources of energy (industrial firewood). Due to their economic importance, researchers are looking for tools that help increase the quality and productivity of the plantations and, with this, they started to make use of molecular markers, in order to obtain information about mainly the genetic diversity among the individuals that compose the population of selection. Thus, in order to contribute to the Pinus taeda breeding program, microsatellite molecular markers (SSR) were used to detect the existence of polymorphisms among 124 plants of a seed orchard. For the development of the study, total genomic DNA was extracted from the plant material (needles) of the 124 plants of Pinus taeda, which were amplified by 5 pairs of microsatellite primers specific to the species. The PCR products were separated on a 6% non-denaturing polyacrylamide gel, stained with silver nitrate and visually analyzed, and on a 3% agarose gel stained with Ethidium Bromide. The microsatellite molecular markers used allowed to analyze the genetic diversity of the Pinus taeda plants evaluated. The diversity information obtained may help in the process of genetic improvement of the species. Depending on the objective of the breeding program, the population and its diversity can be manipulated through crossings and selections.