Photodynamic therapy in endodontic treatment of deciduous teeth: a controlled and randomized trial

Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-04-11T20:50:53Z No. of bitstreams: 1 Camila Basilio Okamoto.pdf: 913886 bytes, checksum: 79173dbe02f659a2461e89c9b40b7912 (MD5) Made available in DSpace on 2022-04-11T20:50:53Z (GMT). No. of bitstreams: 1 Camila Basilio Okamoto.pdf: 913886 bytes, checksum: 79173dbe02f659a2461e89c9b40b7912 (MD5) Previous issue date: 2017-12-19 The success of the treatment is based on the decontamination of the root canal. Thus, efficacious methods have been studied as to the disinfection of the root canal systems (RCS). Of the methods studied, a highlight is the combination of conventional root canal therapy associated with laser and Antimicrobial Photodynamic Therapy (aPDT). The aim of this work was to evaluate bacterial reduction and both clinical and radiographic aspects of the root canal treatment in deciduous teeth when associated with photodynamic therapy. 32 deciduous anterior teeth diagnosed with pulp necrosis were selected. The patients were randomly divided into two groups, which underwent distinct treatments: Group I: conventional root canal treatment (n=15); Group II: conventional root canal treatment associated with aPDT (n=17). For the aPDT, Chimiolux® methylene blue (0.005%) was used as photosensitizer, applied with sterile paper cone into the root canal for 3 minutes, associated with laser application at a wavelength of 660nm (DMC, Laser THERAPY XT – EC), the canal was irradiated for 40 seconds, with the apparatus previously set at energy 4J and power 100mw. Direct contact method has been used at the entrance of the root canal. For the microbiological analysis, two samples of intracanal content were collected with paper cones. One sample was collected before and another immediately after the treatments administered in both groups. Radiographic aspects were evaluated, taking into consideration the recovery process, and we clinically observed fistula and mobility. The evaluations were carried out both 1 and 3 months after treatment. The data obtained went through statistical analysis Mann-Whitney (5%) and showed that G II had 99% bacterial reduction, whereas G I had 93%, though this difference was not statistically significant. Satisfactory tissue recovery was clinically and radiographically observed in both groups. It was therefore possible to conclude that the conventional treatment associated with aPDT was efficacious, however, it did not turn out as superior to conventional treatment alone, as far as this study is concerned. O sucesso do tratamento endodôntico baseia-se na descontaminação do canal radicular. Desta forma, métodos têm sido pesquisados para a obtenção da sanificação do interior do sistema de canais radiculares (SCR) com eficácia; dentre estes, destaca-se a combinação da terapia endodôntica convencional associada ao uso do laser e da terapia fotodinâmica (aPDT). O objetivo deste estudo foi avaliar a redução bacteriana e os aspectos clínicos e radiográficos do tratamento endodôntico em dentes decíduos quando associado à terapia fotodinâmica. Foram selecionados 32 dentes decíduos anteriores com diagnóstico de necrose pulpar. Os pacientes foram divididos aleatoriamente em dois grupos, que receberam tratamentos distintos: Grupo I: tratamento endodôntico convencional (n=15); Grupo II: tratamento endodôntico convencional associado aPDT(n=17). Para aPDT foi utilizado como fotossensibilizador o azul de metileno da marca Chimiolux®, na concentração de 0,005%, aplicado com cone de papel estéril no interior do canal radi cular por 3 minutos, associado a aplicação do laser com comprimento de onda de 660nm (DMC, Laser THERAPY XT), o canal foi irradiado por 40s, com o equipamento previamente calibrado com energia de 4J e potência de 100mW. Foi utilizado o método de contato direto, na entrada do canal radicular. Para análise microbiológica, foram realizadas duas coletas do conteúdo intracanal com cones de papel, uma antes e outra logo após os tratamentos propostos nos dois grupos. Os aspectos radiográficos foram avaliados, considerando o processo de reparação e clinicamente observou-se presença de fístula e mobilidade, as avaliações foram realizadas nos períodos de 1 e 3 meses após o tratamento. Os dados obtidos foram submetidos à análise estatística Mann-Whitney (5%), demonstrando que o Grupo II apresentou 99% de redução bacteriana, enquanto o Grupo I apresentou 93%, porém esta diferença não foi estatisticamente significante. Observou-se reparo satisfatório dos tecidos clínica e radiograficamente nos dois grupos. Concluiu-se que o tratamento convencional associado a aPDT foi eficaz, no entanto não apresentou-se superior ao tratamento convencional unicamente neste estudo.