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Pomen endofitskih gliv za privzem kovin in fiziološko stanje ive (Salix caprea L.) na tleh s prekomernimi koncentracijami kovin
- Publication Year :
- 2019
-
Abstract
- Človek z industrijo in z drugimi dejavnostmi obremenjuje okolje in vpliva na ekosisteme. Dolina dimnikov in okolica Žerjava je močno obremenjena s prekomernimi koncentracijami kovin v tleh. Glavni namen naloge je bil ugotoviti, kako različne vrste izbranih izolatov temnih septiranih endofitov DB146, DB148 in DB149, vplivajo na fiziološko stanje vrbe ive, ki raste na tleh s prekomernimi koncentracijami kovin, ter na njen privzem kovin. Z različnimi metodami smo ovrednotili koncentracije rastlinam dostopnih kovin, fosforja in organske snovi v substratu. Z meritvami koncentracij kovin in fotosinteznih pigmentov v listih smo ovrednotili privzem v rastline in fiziološko stanje ive. Ocenili smo uspešnost kolonizacije posameznih izolatov. Naši rezultati nakazujejo na močan stres, ki verjetno ni posledica prekomernih koncentracij kovin. Stres sta verjetno povečevala pomanjkanje vode ter nutrientov. Višji parametri kolonizacije izolatov DB146 in DB148 nakazujejo na boljšo toleranco teh izolatov na prekomerne koncentracije kovin in na uporabo veziklov za kopičenje in stabilizacijo kovin. Nižji parametri kolonizacije pri izolatu DB149 kažejo na drugačno biologijo in možen negativen vpliv drugih mikroorganizmov. Izsledki naše raziskave nakazujejo, da so izbrani izolati temnih septiranih endofitov, predvsem izolata DB146 in DB148, primerni za souporabo v bioremediaciji. Potrebne so nadaljnje raziskave, da bi dobro razumeli vpliv izbranih glivnih izolatov na splošno fiziološko stanje in na privzem kovin. Industrial activies in Žerjav lead to high accumulation of metals, especially Pb, Cd and Zn) in soils. Our aim was to evaluate contribution of selected isolates of dark septate endophytes on metal accumulation in plants and physiological condition of Salix caprea L. cuttings. We measured bioavailable concentrations of metals, phosphorus and organic matter in substrate. To evaluate physiological condition and metals uptake of cuttings we measured metal concentrations and photosynthetic pigments in leaves. To assess success of colonisation of selected strains we evaluated parameters of colonisation. Goat willow cuttings exhibited strong stress symptoms, which were caused probably not by excessive metal concentrations in substrate. Strong stress factors could be water and nutrient deficiency. High colonisation parameters of isolates DB146 and DB148 are suggesting better tolerance of these isolates on high metal concentrations. Low colonisation parameters of isolate DB149 are suggesting different biology of selected isolate and possible negative effect on growth of native colonisators from substrate. To conclude, our findings suggest isolates, especially DB146 and DB148, are suitable for combined use with goat willows in bioremediation processes. For better understanding of contribution of selected strains on metal uptake and physiological condition of goat willow further research is needed.
Details
- Language :
- Slovenian
- Database :
- OpenAIRE
- Accession number :
- edsair.od......3505..6b18436f2a5f0d9e97eafedf0c3cb5cd