healing potential of Thymbra capitata ethanolic extract

Objectives: In this study, we aimed to detect the chemical compounds of Thymbra capitata ethanolic extract (TC-EtOH) as well as to evaluated its antimicrobial, antioxidant, cytotoxic activities, and in vivo wound healing effects. Methods: The chemical composition of TC-EtOH was analyzed by Gas chromatography-mass spectrometry (GC-MS). Antioxidant and antimicrobial properties were determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH), disc diffusion test and broth micro-dilution (minimal inhibitory concentration [MIC]) methods, respectively. Cytotoxic activity was tested on MG63 (human osteosarcoma) and MCF-7 (human breast carcinoma) cells by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. Tumor necrosis factor alpha (TNF-alpha) protein levels were determined by ELISA. Results: The major components of TC-EtOH were tetratria contane (14.92%), camphor (12.50%), and terpineol (10.77%). TC-EtOH showed powerful antimicrobial activity in C. Tropicalis (0.03 mg/mL). The IC50 values of the TC-EtOH of the DPPH were determined 21.5 mu g/mL. The IC50 values were calculated 37.28 and 44.40 mu g/mL on the MG63 and MCF-7 cell lines, respectively. It was observed that the wounds treated with TC-EtOH showed a faster healing. Conclusions: According to results, T. capitata species are thought to be natural antioxidants and a novel pharmaceutical compound for the pharmaceutical industry. C1 [Hepokur, Ceylan; Misir, Sema] Sivas Cumhuriyet Univ, Fac Pharm, Dept Biochem, TR-58140 Sivas, Turkey. [Tunc, Tutku] Sivas Cumhuriyet Univ, Fac Pharm, Dept Microbiol, TR-58140 Sivas, Turkey. [Tutar, Ugur] Sivas Cumhuriyet Univ, Fac Pharm, Dept Bot, TR-58140 Sivas, Turkey. [Hepokur, Ali Ihsan] NETA OSGB, TR-58000 Sivas, Turkey. [Cicek, Mehmet] Pamukkale Univ, Fac Arts & Sci, Dept Biol, TR-20070 Denizli, Turkey.