Antioxidative properties of selected micromeria speies

U okviru ovog diplomskog rada provedena su dva spektrofotometrijska istraživanja tri odabrane Micromeria vrste hrvatske flore – M. croatica, M. thymifolia i M. juliana. Prvo spektrofotometrijsko istraživanje koje smo proveli određivanje je sposobnosti hvatanja slobodnih radikala dušikovog (II) oksida u usporedbi s poredbenim otopinama aksorbinske i ružmarinske kiseline, te rutina. Sve tri Micromeria vrste pokazale su antioksidacijski potencijal (IC50 81,5-88,1 μg/ml), dok je M. croatica pri najnižoj koncentraciji (81,5 μg/ml) neutralizirala 50% slobodnih radikala. M. thymifolia ipak je neutralizirala preko 70% slobodnih radikala pri većim koncentracijama (400-1600 ug/ml). Drugo spektrofotometrijsko istraživanje koje smo proveli određivanje je sposobnosti inhibicije lipidne peroksidacije u usporedbi s poredbenim otopinoma rutina i ružmarinske kiseline. Sve tri Micromeria vrste pokazale su sposobnost inhibicije (IC50 54,5-206 μg/ml). Najveću antioksidacijsku sposobnost imala je M. croatica koja je već pri koncentraciji od 54,5 μg/ml neutralizirala 50% slobodnih radikala. Two spectrophotometrical researches of three Micromeria species of Croatian flora – M. croatica, M. thymifolia and M. juliana where examined in this thesis. First spectrophotometrical research we examined was capacity of inhibition of free NO(II) radicals compared to ascorbic acid, rosmarinic acid and rutin. All three Micromeria species expressed antioxidative capacity (IC50 81,5 – 88,1 μg/ml), while M. croatica had neutralized 50% of free radicals at lowest concentration (81,5 μg/ml). M. thymifolia had neutralized over 70% of free radicals at highest levels of concentration (400-1600 μg/ml). Second spectrophotometrical research we conducted was to determine capacity of inhibition of lipid peroxidation in comparison to rutin and rosmarinic acid. All three Micromeria species expressed antioxidative capacity (IC50 54,5-206 μg/ml). M. croatia had highest capacity of three examined species and had neutralized over 50% of free at concentration of 54,5 μg/ml.