POSTER: Epitope-mapping of antiviral nanobodies against poliovirus

Nanobodies are recombinant single-domain antibody fragments deriving from heavy-chain antibodies (HCAb) found in camelids. Since the antigen-binding site of the camelid HCAb is comprised in one single domain, the nanobody harbors the full antigen-binding capacity of naturally occurring HCAb. Because of their characteristic structure, nanobodies have the tendency to bind into cavities. In this study the epitopes of five neutralizing nanobodies against poliovirus type 1 were mapped on the deduced amino acid sequence of the capsid coding region from viral neutralizing-escape variants.