How metabolomics is used to support the MetaPath solution through metabolic profiling of cheese fermentation industry ecosystem models ?

International audience; Consumer expectations and societal evolutions are at the heart of the concerns of fermentation professionals. The organoleptic properties of their products are closely correlated to the manufacturing process but also to its composition specifically the microbial ecosystems introduced to produce this fermentation. In this context, the Bpifrance MetaPath project aims to reconstruct the metabolic maps of these microbial ecosystems in order to guide the industrialist in the selection of microbial strains at the time of product design. As a partner, the MetaToul platform aims to perform metabolic profiling of ecosystems in the cheese model of industrial partner Bel. The sample preparation and analysis methods developed on the MetaToul platform on this complex matrix allow a complete and precise study of the central and energetic metabolism (~80 metabolites) of these ecosystem models. It was validated with the addition of IDMS on repeatability parameters, extraction yield and matrix effect. The samples produced for metabolomics are analyzed on the following analytical systems: ion chromatography coupled to an LTQ-Orbitrap-Velos (Thermo) for the analysis of central energetic metabolism, liquid chromatography coupled to a QExactive+ (Thermo) for the analysis of amino acids, coenzymes A and the mevalonate pathway. The metabolic profiling data produced on the different ecosystem models of the partner will be integrated with others omics data into the MetaPath solution by Abolis/ Microbiome Studio partner in order to reconstruct active metabolic maps, true identity cards of the metabolic activity of microbial ecosystems in the fermentation of cheese matrix.