Monitoring of drinking water quality at Ferrara city: study of metabolic perturbation in Cyprinus Carpio

MONITORING OF DRINKING WATER QUALITY AT FERRARA CITY: STUDY OF METABOLIC PERTURBATION IN CYPRINUS CARPIO: Fabio Vivarelli, Donatella Canistro, Andrea Sapone, Moreno Paolini. In the global pollution scenario, a latent, and therefore insidious danger is the presence of toxic chemicals in drinking water, derived, paradoxically, from the disinfection process which is compulsory to protect public health from diseases. In most countries, the surface water is disinfected by chloro-derivatives which react with the dissolved organic matter (humic and fulvic acids), giving rise to the formation of carcinogenic organo-chlorinated by-products. Many of them, however, such as dichloroacetic acid and chloroform are not mutagens and act through epigenetic mechanisms. It is not to be underestimated the risk of gastrointestinal and urogenital tumors related to chronic exposure to these compounds, emerged from epidemiological investigations. This study is aimed to evaluate the possible effects on xenobiotic metabolism of fish (Cyprinus carpio) exposed to Ferrara city drinking water. It is known that the up-regulation of cytochrome P450 (CYP450) superfamily is associated with non-genotoxic (epigenetic) outcomes such as co-carcinogenesis (increasing bioactivation) and promotion (overgeneration of reactive free radicals). C. carpio, widely distributed in rivers and lakes, was recently considered an effective biomarker recommended by the EU to assess the toxic effects of chemicals in the aquatic environment. Animals were treated daily (3 or 6 consecutive days) intraperitoneally with concentrated drinking water collected from the distribution networks of the city, at 3L/eq dosage. The evaluation of metabolic perturbation has been performed in the hepatopancreas subcellular preparation. Four sampling points were selected: F-raw river water, A-water after treatment, R1 and R2-water from two points chosen along the network. After 3 day treatment, a marked and significant (p