Bazı Kışlık ve Yazlık Buğday Genotiplerinde Tahil Kist Nematoduna Dayanıklıklık Sağlayan Cre1 ve Cre3 Genlerinin Tespiti

Breeding for resistance to the cereal cyst nematodes (CCN) Heterodera filipjevi (Madzhidov) Stelter, and H. avenae (Wollenweber) is presently being undertaken by breeding programs at research institutions and university in Turkey. So far, most work indicated that predominant species in Turkey is closely related H. avenae, H. filipjevi and H. latipons. Cre1 and Cre3 genes are known the most common resistant genes to provide resistance against cyst nematodes. In vitro resistance assaying for this species indicates that Cre1 is a moderate source of resistance against the H. filipjevi population, whereas Cre3 gene appears ineffective. In current study, different combination of primers (Creco and G035) amplifying Cre1gene and the primer xgwm301-2d amplifying Cre3 gene have been investigated whether some winter and summer in wheat genotypes carry those resistance genes. The results indicated that Crecon primers of Cre1 gene are amplifiable for all wheat genotypes, whereas other primer sets, primer set of Xgwm301-2d belong to Cre3 gene and the primer set G035 belong to Cre1 gene did not produce expected amplicons. The results imply that Cre3 gene is absent in wheat genomes screened whereas, further study is needed for conclusive results for Cre1 gene. TÜBİTAK 214O419