[Effect of Negative Regulation of LncRNA XIST to MiR-196b on the Biological Behavior of Acute Myeloid Leukemia Cells KG1a]

To investigate the effect and molecular mechanism of lncRNA X-inactive specific transcript (XIST) on the proliferation and apoptosis of acute myeloid leukemia cells KG1a.Forty-one patients with acute myeloid leukemia from January 2017 to May 2019 treated in Beijing Aerospace Center Hospital were collected, as well as 20 patients who conformed to the international standard of iron deficiency anemia as control group. KG1a cells were divided into pcDNA group, pcDNA-XIST group, pcDNA-XIST+miR-NC group, and pcDNA-XIST+miR-196b group. Real-time fluorescence quantitative PCR was used to detect the expressions of XIST and miR-196b, CCK-8 was used to detect cell activity, flow cytometry was used to detect cell cycle and apoptosis, Western blot method was used to detect the protein expressions of cleaved-caspase3, pro-caspase3, Bax, and Bcl-2, and dual luciferase report experiment was used to detect the targeting relationship between XIST and miR-196b.The expression level of lncRNA XIST in bone marrow cells in the AML group was significantly lower than that in the iron deficiency anemia group (P0.001). Compared with pcDNA group, the expression level of lncRNA XIST, proportion of cells in GOverexpression of lncRNA XIST can inhibit the proliferation of acute myeloid leukemia cells KG1a and promote apoptosis by down-regulating the expression of miR-196b.LncRNA XIST负调控miR-196b对急性髓系白血病细胞KG1a生物行为的影响.探讨lncRNA X染色体失活特异性转录因子（XIST）对急性髓系白血病细胞KG1a增殖和凋亡的影响及其相关作用机制。.收集北京航天中心医院2017年1月至2019年5月收治的41例急性髓系白血病患者，以及20例符合缺铁性贫血国际标准的患者作为对照组。将KG1a细胞分为pcDNA、pcDNA-XIST、pcDNA-XIST+miR-NC和pcDNA-XIST+miR-196b共4组。实时荧光定量PCR检测XIST和miR-196b表达水平，CCK-8检测细胞活性，流式细胞术检测细胞周期和细胞凋亡，Western blot法检测cleaved-caspase3、pro-caspase3、Bax、Bcl-2蛋白表达，双荧光素酶报告实验检测XIST和miR-196b的靶向关系。.急性髓系白血病组患者骨髓细胞中lncRNA XIST表达水平明显低于缺铁性贫血组（P0.001）。KG1a细胞pcDNA-XIST组lncRNA XIST表达水平、G过表达lncRNA XIST通过下调miR-196b表达抑制急性髓系白血病细胞KG1a增殖，促进细胞凋亡。.