[Growth factors promote the survival and proliferation of human spermatogonium stem cells in vitro]

To study the effects of growth factors on the survival and proliferation of human spermatogonial stem cells (SSCs) in vitro.SSCs were treated with the growth factors SCF, LIF and bFGF added to the culture, each at the concentrations of 0, 5, 10 and 20 microg/L and repeated three times. The survival time and proliferation rate of the cells were determined every 8-12 hours and their morphological features observed with the light microscope and electron microscope.The survival time and proliferation rate of the SSCs were significantly increased in the treated groups as compared with the control (P0.05).The growth factors SCF, LIF and bFGF can promote the survival and proliferation of SSCs in vitro.