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Involvement of nuclear factor-1 (NF1) binding motif in the regucalcin gene expression of rat kidney cortex: the expression is suppressed by cisplatin administration
- Source :
- Molecular and cellular biochemistry. 219(1-2)
- Publication Year :
- 2001
-
Abstract
- The binding of nuclear factor on the promoter region of the regucalcin gene and the expression of regucalcin in the kidney cortex of rats was investigated. Nuclear extracts from kidney cortex were used for oligonucleotide competition gel mobility shift assay. An oligonucleotide between position -523 and -506 in the 5'-flanking region of the rat regucalcin gene, which contains a nuclear factor 1 (NF1) consensus motif TTGGC(N)6CC, competed with the probe for the binding of the nuclear protein from kidney cortex. The mutation of TTGGC in the consensus sequence caused an inhibition of the binding of nuclear factors. The binding of nuclear factor on the 5'-flanking region was clearly reduced in the kidney cortex obtained at 1, 2, and 3 days after a single intraperitoneal administration of cisplatin (1.0 mg/100 g body wt) to rats. Moreover, cisplatin administration caused a remarkable decrease in regucalcin mRNA levels and regucalcin concentration in the kidney cortex. Also, serum regucalcin concentration was significantly decreased by cisplatin administration. Meanwhile, serum urea nitrogen concentration was markedly elevated by cisplatin administration. The present study demonstrates that the specific nuclear factor binds to the NF1-like sequence in the promotor region of regucalcin gene in the kidney cortex of rats, and that the nuclear factor binding and regucalcin expression are suppressed by cisplatin administration.
- Subjects :
- Male
Kidney Cortex
Time Factors
Amino Acid Motifs
Molecular Sequence Data
Binding, Competitive
Consensus Sequence
Animals
Rats, Wistar
Promoter Regions, Genetic
Binding Sites
Base Sequence
Calcium-Binding Proteins
Intracellular Signaling Peptides and Proteins
Rats
DNA-Binding Proteins
NFI Transcription Factors
Gene Expression Regulation
Mutation
CCAAT-Enhancer-Binding Proteins
Kidney Diseases
Y-Box-Binding Protein 1
Cisplatin
Sulfotransferases
Carboxylic Ester Hydrolases
Transcription Factors
Subjects
Details
- ISSN :
- 03008177
- Volume :
- 219
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular biochemistry
- Accession number :
- edsair.pmid..........0afe5276af2cb5936302f5800a7109d4